a peptide corresponding to amino acids 312-340 of napi2b  (Bio-Synthesis Inc)

Journal: Cancer Immunity : a Journal of the Academy of Cancer Immunology

Article Title: Monoclonal antibody MX35 detects the membrane transporter NaPi2b (SLC34A2) in human carcinomas

doi:

Figure Lengend Snippet: MX35 epitope mapping. (A) Schematic diagram of the predicted membrane topology of the sodium-dependent phosphate transporter 2b protein. (B) Schematic presentation of the NaPi2b protein and fusion proteins derived from the potentially largest extracellular region (aa 188-361) and the location of the MX35 epitope. (C) NaPi2b fusion proteins expressed in bacteria and immunoblotted with mAb MX35 after separation by SDS-PAGE.

Article Snippet: Peptide blocking and peptide ELISA A peptide corresponding to amino acids 312-340 of NaPi2b was chemically synthesized (Bio-Synthesis, Inc. Lewisville, TX) and co-incubated at varying concentrations with mAb MX35 prior to assaying mAb MX35 binding to MX35-positive and MX35-negative cells by MHA.

Techniques: Derivative Assay, SDS Page

Journal: Cancer Immunity : a Journal of the Academy of Cancer Immunology

Article Title: Monoclonal antibody MX35 detects the membrane transporter NaPi2b (SLC34A2) in human carcinomas

doi:

Figure Lengend Snippet: Monoclonal antibody MX35 epitope mapping using a series of synthetic peptides located in a putative extracellular domain of the NaPi2b protein.

Article Snippet: Peptide blocking and peptide ELISA A peptide corresponding to amino acids 312-340 of NaPi2b was chemically synthesized (Bio-Synthesis, Inc. Lewisville, TX) and co-incubated at varying concentrations with mAb MX35 prior to assaying mAb MX35 binding to MX35-positive and MX35-negative cells by MHA.

Techniques:

Journal: Cancer Immunity : a Journal of the Academy of Cancer Immunology

Article Title: Monoclonal antibody MX35 detects the membrane transporter NaPi2b (SLC34A2) in human carcinomas

doi:

Figure Lengend Snippet: NaPi2b/MX35 antigen biochemical characterization. Monoclonal antibody MX35 immune precipitations from metabolically-labeled SK-RC-18 cell lysates separated by SDS-PAGE under reducing conditions and visualized by autoradiography. (A) Enzymatic deglycosylation analysis of 35S-labeled immune complexes. (B) Enzymatic deglycosylation analysis of immune complexes derived from 3H-glucosamine labeled cells. (C) Acylation analysis of immune complexes from 3H-palmitate labeled cells.

Article Snippet: Peptide blocking and peptide ELISA A peptide corresponding to amino acids 312-340 of NaPi2b was chemically synthesized (Bio-Synthesis, Inc. Lewisville, TX) and co-incubated at varying concentrations with mAb MX35 prior to assaying mAb MX35 binding to MX35-positive and MX35-negative cells by MHA.

Techniques: Metabolic Labelling, Labeling, SDS Page, Autoradiography, Derivative Assay

Journal: Cancer Immunity : a Journal of the Academy of Cancer Immunology

Article Title: Monoclonal antibody MX35 detects the membrane transporter NaPi2b (SLC34A2) in human carcinomas

doi:

Figure Lengend Snippet: MX35 epitope mapping. (A) Schematic diagram of the predicted membrane topology of the sodium-dependent phosphate transporter 2b protein. (B) Schematic presentation of the NaPi2b protein and fusion proteins derived from the potentially largest extracellular region (aa 188-361) and the location of the MX35 epitope. (C) NaPi2b fusion proteins expressed in bacteria and immunoblotted with mAb MX35 after separation by SDS-PAGE.

Article Snippet: A peptide corresponding to amino acids 312-340 of NaPi2b was chemically synthesized (Bio-Synthesis, Inc. Lewisville, TX) and co-incubated at varying concentrations with mAb MX35 prior to assaying mAb MX35 binding to MX35-positive and MX35-negative cells by MHA.

Techniques: Derivative Assay, SDS Page

Journal: Cancer Immunity : a Journal of the Academy of Cancer Immunology

Article Title: Monoclonal antibody MX35 detects the membrane transporter NaPi2b (SLC34A2) in human carcinomas

doi:

Figure Lengend Snippet: Monoclonal antibody MX35 epitope mapping using a series of synthetic peptides located in a putative extracellular domain of the NaPi2b protein.

Article Snippet: A peptide corresponding to amino acids 312-340 of NaPi2b was chemically synthesized (Bio-Synthesis, Inc. Lewisville, TX) and co-incubated at varying concentrations with mAb MX35 prior to assaying mAb MX35 binding to MX35-positive and MX35-negative cells by MHA.

Techniques:

Journal: Cancer Immunity : a Journal of the Academy of Cancer Immunology

Article Title: Monoclonal antibody MX35 detects the membrane transporter NaPi2b (SLC34A2) in human carcinomas

doi:

Figure Lengend Snippet: NaPi2b/MX35 antigen biochemical characterization. Monoclonal antibody MX35 immune precipitations from metabolically-labeled SK-RC-18 cell lysates separated by SDS-PAGE under reducing conditions and visualized by autoradiography. (A) Enzymatic deglycosylation analysis of 35S-labeled immune complexes. (B) Enzymatic deglycosylation analysis of immune complexes derived from 3H-glucosamine labeled cells. (C) Acylation analysis of immune complexes from 3H-palmitate labeled cells.

Article Snippet: A peptide corresponding to amino acids 312-340 of NaPi2b was chemically synthesized (Bio-Synthesis, Inc. Lewisville, TX) and co-incubated at varying concentrations with mAb MX35 prior to assaying mAb MX35 binding to MX35-positive and MX35-negative cells by MHA.

Techniques: Metabolic Labelling, Labeling, SDS Page, Autoradiography, Derivative Assay